Bezmialem Science Koleksiyonu
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Browsing Bezmialem Science Koleksiyonu by Subject "antioxidant"
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Publication Open Access Biological activities and chemical composition of Turkish sweetgum balsam (Styrax liquidus) essential oil(2022-01-01T00:00:00Z) BÜYÜKKILIÇ ALTINBAŞAK, BETÜL; İSSA, GHASSAN; ZENGİN KURT, BELMA; DEMİRCİ, BETÜL; BÜYÜKKILIÇ ALTINBAŞAK, BETÜL; ZENGİN KURT, BELMAObjective: The purpose of this present study was to make a chemical analysis of the composition of essential oil obtained from sweetgum balsam and to examine its antimicrobial, anticholinesterase, α-glucosidase inhibition, and antioxidant activities. Methods: The essential oil obtained by the hydrodistillation method was analyzed by gas chromatography-mass spectrometry systems. The antimicrobial activities of the essential oil were evaluated by disc diffusion and resazurin microplate methods, the anticholinesterase effect was determined by using in vitro AChE and BChE enzymes inhibition assays, and the antioxidant effect was evaluated by ABTS and CUPRAC methods. Results: The main components of the essential oil were determined as styrene (92.6%) and α-pinene (2.2%). The essential oil showed weak antimicrobial activity against K. pneumoniae, S. epidermidis but strong antimicrobial activity against A. baumannii, C. glabrata. It showed moderate inhibitory activity to AChE and BChE enzymes, and IC50 values were calculated as 36.5 μg/mL and 69.5 μg/mL, respectively. It also showed low inhibition of α-glucosidase (IC50 value was 637.2 μg/mL) and a similar antioxidant effect in the CUPRAC and ABTS method (A0.5 value was 637.2 μg/mL and IC50 value was 632.2 μg/mL, respectively). Conclusion: Styrax Liquidus essential oil can be considered a natural antimicrobial agent due to its strong antimicrobial activity capacity against A. baumannii and C. glabrata strainsPublication Open Access Investigation of Bioactive Components, Antioxidant and Antimicrobial Activities of Traditional Turkish Beverage Hardaliye(2022-01-01T00:00:00Z) POLAT SARI, SİLVA; DİNÇ, HARİKA ÖYKÜ; BÜYÜKKILIÇ ALTINBAŞAK, BETÜL; YÜKSEL MAYDA, PELİN; AKGÜL, ÖZER; SAPMAZ, BURCU; ÖNER, YAŞAR ALİ; ÇALIŞKAN, REYHAN; DİNÇ, HARİKA ÖYKÜ; BÜYÜKKILIÇ ALTINBAŞAK, BETÜLObjective: In our study, it was aimed to make the chemical analysis of hardaliye products (H1 and H2), which are commercially available by different manufacturers, and to examine their antioxidant and antimicrobial activities. Methods: Antioxidant activity, organic acid and phenolic compounds, and antimicrobial activity in Hardaliye products (H1 and H2) were determined by using 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, [liquid chromatographyhigh resolution mass spectrometry (LC-HR/MS)] and liquid microdilution methods, respectively. Results: Ascorbic acid and fumaric acid from organic acids were determined by chemical analysis of hardaliye samples by LC-HR/ MS method. While ascorbic acid concentrations were 21.295 mg/L and 26.84 mg/L in H1 and H2, respectively, fumaric acid concentrations were 59.55 mg/L in H1, and 224.562 mg/L in H2. While the phenolic component with the highest concentration in H1 was resveratrol (44.57 mg/L), it was observed that the phenolic component with the highest concentration in H2 was p-coumaric acid (31.87 mg/L). In terms of antioxidant activity, diphenyl-2- picrylhydrazylfree radical scavenging activity of hardaliye samples was determined as 2.07±0.004% and 2.49±0.004% in H1 and H2, respectively. It was determined that hardaliye samples showed inhibitory effect [H1 minimum inhibitor concentration (MIC): 15.625 µg/mL, H2 MIC: <3.9 µg/mL] against only S. Epidermidis ATCC 49461 strains among the tested microorganisms. Conclusion: In our study, it was determined that two different commercial Hardaliye products contained very low concentrations of phenolic compounds compared to the data in the literature, and therefore it was thought that tested Hardaliye products did not show antioxidant activity.Publication Open Access Is high Dose Intravenous Vitamin C Safe to Use in SARSCoV-2 Treatment?(2020-06-01T00:00:00Z) Koçyiğit, Abdürrahim; KOÇYİĞİT, ABDÜRRAHİMSevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2) disease which occurred in China in late 2019 and caused pandemia is an important public health problem. The virus has been found to be a member of the beta-coronavirus family of the same species as the SARS-CoV and SARS-related bat CoV's. The way it spreads indicates that SARS-CoV-2 can be transmitted from person to person and be more contagious than SARS-CoV. In general, SARS-CoV-2 is an acute disease, but it can be fatal and its mortality is around 2-3%. Acute respiratory distress syndrome (ARDS) is the most important cause of death. Due to cytokines storm, hyperinflammation is a distinctive feature of ARDS, leading to cellular injury, organ failure and death. A fast, accessible, effective and safe treatment is required to save lives and reduce spreading. However, there is still no vaccine or drug developed for the prevention or definitive treatment of SARS-CoV-2. In addition to broad-spectrum antivirals and some other substances for the treatment of the disease, high-dose intravenous (i.v.) vitamin C (VC) is also recommended to take advantage of the antiviral and antioxidant effect. However, it has a pro-oxidant effect rather than an antioxidant. The cumulative effect of oxidative stress caused by inflammation and VC, besides the antiviral effect, can cause serious inflammation and oxidative damage to the tissues. In this review, the function, antiviral efficacy and possible negative consequences of high dose i.v. VC recommended for SARS-CoV2 treatment will be discussed in the light of the literature.Publication Open Access Serum Melatonin Levels in Patients with Sudden Sensorineural Hearing Loss(2020-07-01T00:00:00Z) Baki, Ahmet; ÖZER, Ömer Faruk; Yildiz, Muhammet; KÖKTAŞOĞLU, FATMANUR; ÖZER, ÖMER FARUK; KÖKTAŞOĞLU, FATMANURObjective: This study aimed to investigate the serum melatonin levels of patients with idiopathic sudden sensorineural hearing loss (ISSNHL). Methods: This study enrolled 22 patients with ISSNHL and 22 asymptomatic healthy volunteers. The subjects underwent pure tone audiometry and serum melatonin levels were measured. Results: The patient group was composed of 12 women (mean age, 46.41 +/- 7.8) and 10 men (mean age, 48 +/- 6.46), and the control group was composed of 12 women (mean age, 40.25 +/- 5.28) and 10 men (mean age, 38.4 +/- 27.08). The serum melatonin levels were significantly lower in the patient group than in the control group (p=0.001). A statistically significant strong negative correlation was found between serum melatonin level and severity of hearing loss (r=-0.644; p=0.001). Conclusion: Serum melatonin levels of patients with ISSNHL were lower than controls. A statistically significant strong negative correlation was found between serum melatonin level and the severity of hearing loss.